Premium
Stem‐cell factor in aplastic anemia: in vitro expression in bone marrow stroma and fibroblast cultures
Author(s) -
Krieger M. Slanicka,
Nissen C.,
WodnarFilipowicz A.
Publication year - 1995
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1995.tb00681.x
Subject(s) - stroma , stem cell factor , stromal cell , bone marrow , aplastic anemia , biology , basic fibroblast growth factor , leukemia inhibitory factor , stem cell , growth factor , cancer research , chemistry , microbiology and biotechnology , immunology , haematopoiesis , cytokine , interleukin 6 , biochemistry , receptor , immunohistochemistry
In vitro expression of stem‐cell factor (SCF) by bone marrow (BM) cells of 30 patients with aplastic anemia (AA) has been analyzed at the mRNA and protein levels. While no deficiencies were found in SCF mRNA expression, low levels of soluble SCF protein were measured in poorly growing AA stroma cultures. The SCF protein concentration in the supernatant and the confluence of AA stroma growth were found to correlate (R = 0.70). Defective proliferation was observed in the majority (20/30) of AA stroma cultures and was paralleled by poor growth of homogeneous cultures of fibroblasts from the same marrow sample. AA stroma growth was enhanced by addition of exogenous SCF in combination with interleukin‐11 (IL‐11), leukemia inhibitory factor (LIF), and basic fibroblast growth factor (bFGF). Our results demonstrate that deficient growth of stroma cells results in decreased production of SCF. Therefore, SCF and other stroma‐derived cytokines may be of therapeutic value in AA patients with documented defects within the BM microenvironment.