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Hematologic effects of stem cell factor (SCF) and leukemia inhibitory factor (LIF) in vivo : LIF‐induced thrombocytosis in SCF‐primed mice
Author(s) -
Ulich Thomas R.,
Castillo Juan,
Shin Sung S.,
Yin Songmei,
Duryea Diane,
Tarpley John,
Samal Babru
Publication year - 1995
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1995.tb00675.x
Subject(s) - thrombocytosis , leukemia inhibitory factor , stem cell factor , in vivo , cancer research , stem cell , leukemia inhibitory factor receptor , medicine , leukemia , inhibitory postsynaptic potential , immunology , platelet , biology , haematopoiesis , cytokine , interleukin 6 , microbiology and biotechnology , genetics
Stem cell factor (SCF) administered as daily bolus injections in dose‐response experiments in mice causes a progressive and dramatic dose‐dependent panleukocytosis characterized by neutrophilia, eosinophilia, monocytosis, and lymphocytosis. SCF causes circulating platelet numbers to be dose‐dependently increased after 2 weeks of daily injections. Leukemia inhibitory factor (LIF) administered as daily bolus injections in mice causes a peripheral leukopenia that is largely due to peripheral lymphopenia. LIF causes thrombocytosis peaking after approximately 1 w. Coinjection of SCF and LIF for 1 to 2 wk in mice does not cause a much greater thrombocytosis than the maximum thrombocytosis achievable with SCF or LIF alone. On the other hand, daily injection of SCF for 5 days followed by daily injection of LIF for 5 to 6 d in mice causes a very substantial increase in platelets that was lineage‐specific in terms of not being accompanied by a generalized leukocytosis. In contrast, only a very modest thrombocytosis was noted in SCF‐primed LIF‐treated rats. LIF causes a large increase in the cytoplasmic volume of splenic megakaryocytes in mice, but not in rats. In conclusion, SCF‐induced priming followed by LIF‐induced maturation of megakaryocytes causes a substantial selective increase in the numbers of circulating platelets in mice.