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Assessment of minimal residual disease in acute promyelocytic leukaemia with t(15;17) by chromosome painting
Author(s) -
Temperani P.,
Vaccari P.,
Giacobbi F.,
Gandini G.,
Ferrari M. G.,
Emilia G.
Publication year - 1995
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1995.tb00226.x
Subject(s) - chromosomal translocation , fluorescence in situ hybridization , metaphase , acute promyelocytic leukemia , chromosome , minimal residual disease , fish <actinopterygii> , biology , microbiology and biotechnology , pathology , retinoic acid , cancer research , medicine , leukemia , genetics , gene , fishery
To detect the minimal residual disease (MRD) in acute promyelocytic leukaemia patients treated with all‐trans retinoic acid, we compared the sensitivity of metaphase fluorescence in situ hybridization (FISH) with conventional analysis of G‐banded metaphases. 5 out of 6 patients studied at diagnosis showed the t(15;17) translocation. 4 out of 5 patients carrying t(15;17) achieved complete remission and conventional cytogenetic conversion. In 3 cases the whole chromosome painting (WCP) probe 17 discovered one normal chromosome 17 and two fragments indicative of t(15;17) persistence. The FISH‐WCP technique seems to be highly sensitive and recommendable in monitoring leukaemias with specific chromosome rearrangements.