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Immunocytochemical detection of bone marrow‐invasive neuroblastoma cells
Author(s) -
Nagai Junichi,
Kigasawa Hisato,
Tomioka Kayoko,
Koga Noriko,
Nishihira Hirokazu,
Nagao Takeshi
Publication year - 1994
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1994.tb01868.x
Subject(s) - bone marrow , neuroblastoma , immunocytochemistry , pathology , monoclonal antibody , staining , microbiology and biotechnology , giemsa stain , peripheral blood mononuclear cell , biology , cell culture , antibody , medicine , immunology , in vitro , biochemistry , genetics
We evaluated the utility of an immunocytochemical technique employing the commercially available anti‐CD56 monoclonal antibody, NKH 1. The utility and sensitivity of this technique in the detection of invasive neuroblastoma (NB) cells in the bone marrow were compared with those of Wright‐Giemsa staining. The correlation coefficient for the percent NB cells detected using Wright‐Giemsa staining with the percent NKH 1 immunoreactive cells was 0.78. In the analysis of specificity, this monoclonal antibody showed slight cross‐reactivity with normal bone marrow cells, including macrophages, lymphocytes and osteoblasts. In the evaluation of the sensitivity of the NKH 1 immunocytochemical technique, SK‐N‐DZ and SK‐N‐SH NB cell lines were added to morphologically normal bone marrow mononuclear cells from patients without NB to the final NB cell line at concentrations of 2%, 1% and 0.1%. NB cells at the final concentration of 0.1 % could be detected by the immunocytochemical technique. We conclude that the NKH 1 immunocytochemical staining technique is useful in the detection of metastatic NB cells in bone marrow.