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Involvement of HIV nef protein in abnormal hematopoiesis in AIDS: in vitro study on bone marrow progenitor cells
Author(s) -
Calenda V.,
Graber P.,
Delamarter JF.,
Chermann JC.
Publication year - 1994
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1994.tb01294.x
Subject(s) - haematopoiesis , progenitor cell , bone marrow , in vitro , human immunodeficiency virus (hiv) , progenitor , medicine , immunology , cancer research , biology , stem cell , virology , microbiology and biotechnology , genetics
In previous studies (1–4), we described disrupted hematopoiesis in long‐term human bone marrow cultures infected with HIV. Conditioned medium (CM) from HIV‐1 non‐productively infected liquid cultures inhibits the proliferation of granulo‐monocytic progenitor cells (CFU‐GM) in clonogenic assays. A growth‐inhibitory soluble factor was therefore suspected and we report here that this factor has been identified as the nef gene product. These nef ‐containing supernatants are specifically neutralized by anti‐ nef antibodies and recombinant nef has the same growth inhibitory properties. We used a nef ‐deficient virus to confirm that nef protein is responsible for the in vitro growth inhibition of CFU‐GM.