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Reactive oxygen species of neutrophils from patients with monosomy 7 in the bone marrow: Contradictory chemiluminescence activity by whole blood or by purified cells
Author(s) -
Ristola M.,
Kere J.,
Ruutu T.,
Repo H.
Publication year - 1994
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1994.tb01281.x
Subject(s) - chemiluminescence , bone marrow , reactive oxygen species , luminescent measurements , chemistry , monosomy , medicine , whole blood , pathology , immunology , biochemistry , chromatography , karyotype , materials science , chromosome , gene , optoelectronics , luminescence
Monosomy 7, a deletion of the long arm of chromosome 7, was shown in the neutrophils of peripheral blood in 5 of 6 patients with a myelodysplastic syndrome or leukemia who had monosomy 7 in their bone marrow cells. In a chemiluminescence assay the production of reactive oxygen species by neutrophils from patients was increased in whole blood and decreased in purified cells, which suggests that neutrophils with monosomy 7 tolerate poorly the cell purification procedures used. The in vitro migration of purified neutrophils obtained from patients with monosomy 7 was impaired. It is known that patients with monosomy 7 have an increased susceptibility to infections. It is possible that neutrophils with monosomy 7 are too easily triggered to a full‐scale respiratory burst and thereby the cells exhaust their ability to eliminate invading microbes efficiently.