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Effects of soluble interleukin‐1 receptor and tumor‐necrosis factor receptor, respectively, on the IL‐1‐ and TNF‐α‐induced DNA synthesis of acute myeloblastic leukemia blasts in vitro
Author(s) -
Carter Anna,
Haddad Nuhad,
Draxler Ilana,
Tatarsky Ilana
Publication year - 1994
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1994.tb00177.x
Subject(s) - tumor necrosis factor alpha , receptor , acute myeloblastic leukemia , cytokine , in vitro , dna synthesis , interleukin , leukemia , biology , microbiology and biotechnology , immunology , cancer research , chemistry , medicine , endocrinology , biochemistry
This study demonstrates that soluble interleukin‐1 receptor and tumor necrosis factor receptor modulate their corresponding cytokine‐induced DNA synthesis of acute myeloblastic leukemia (AML) blasts in a dose‐dependent, bimodal fashion; at lower concentrations they enhanced, while at high concentrations they inhibited, the cytokine‐mediated effects. Furthermore, the concentrations of endogenously produced IL‐1β and TNF‐a were found to be significantly (p<0.01) higher in supernatants of AML cells cultured in the presence of corresponding soluble receptors compared to their levels in supernatants of cells growing in the absence of these molecules. Our data might suggest that the attenuation of the spontaneous decay of IL‐1β as well as TNF‐a activities by soluble receptors may account for their ability to augment some of their effects.