Premium
G‐CSF enhances the immunoglobulin generation rather than the proliferation of human B lymphocytes
Author(s) -
Morikawa Keiko,
Miyawaki Toshio,
Oseko Fumimaro,
Morikawa Shigeru,
Imai Katsuyuki
Publication year - 1993
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1993.tb00615.x
Subject(s) - antibody , polyclonal antibodies , microbiology and biotechnology , flow cytometry , b cell , biology , in vitro , cell culture , immunoglobulin g , granulocyte , chemistry , immunology , biochemistry , genetics
The effect of granulocyte‐colony stimulating factor (G‐CSF) on human B‐cell function was studied in in vitro cultures. G‐CSF alone had no effect on the proliferative response of resting B cells, but it slightly enhanced the proliferative response of these cells in the presence of polyclonal B‐cell mitogen, Staphylococcus aureus Cowan strain I (SAC) at concentrations of 0.2 to 25 μg/ml (1.5‐fold increase in the DNA synthesis). In contrast, immunoglobulin (Ig) secretion of activated B cells was increased approximately three‐fold to four‐fold by adding G‐CSF to the cultures. The neutralization of G‐CSF bioactivity with anti‐G‐CSF antibody abrogated this effect. Though cytoplasmic Ig‐positive cells or plasma cell marker‐positive cells did not change, the expression of IgM mRNA in antibody‐producing B cells increased in the presence of G‐CSF in the cultures. Interestingly, human B lymphocytes are shown to express the binding to biotin‐conjugated G‐CSF preparation, but not to biotin‐conjugated GM‐CSF preparation when examined by flow cytometry. These data suggest that G‐CSF may influence B‐cell function in special circumstances.