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The in vitro effects of interferon‐gamma, interferon‐alpha, and tumour‐necrosis factor‐alpha on erythroid burst‐forming unit growth in patients with non‐leukaemic myeloproliferative disorders
Author(s) -
Kanfer Edward J.,
Price Cathy M.,
Gordon Andrea A.,
Barrett A. John
Publication year - 1993
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1993.tb00157.x
Subject(s) - in vitro , erythropoietin , haematopoiesis , tumor necrosis factor alpha , alpha interferon , interferon , medicine , interferon gamma , growth inhibition , interferon alfa , myeloproliferative disorders , cytokine , alpha (finance) , growth factor , endocrinology , immunology , biology , stem cell , receptor , biochemistry , genetics , construct validity , nursing , patient satisfaction
We have studied the effects of interferons gamma (IFN‐γ) and alpha (IFN‐α), and tumour‐necrosis factor‐alpha (TNF) on circulating 14‐day erythroid progenitor cell (BFU‐E) growth in vitro from patients with non‐leukaemic myeloproliferative disorders (MPD) compared with normal controls. IFN‐γ (1000 U/ml) inhibited BFU‐E growth in all controls studied (mean growth ± SE = 61 % ± 6%, n = 10). In 7 of 11 MPD studied there was no inhibition, and in some cases clear enhancement of BFU‐E growth by IFN‐γ. When cultured in the presence of recombinant erythropoietin (rEpo) 1 U/ml, both IFN‐α and TNF (at 100 and 1000 U/ml) produced a similar degree of inhibition of BFU‐E growth in MPD and controls. The inhibition by 100 U/ml IFN‐α was abrogated, partially in controls but completely in MPD, by increasing the dose of rEpo to 5 U/ml. Similarly, the increase in rEpo dose enhanced BFU‐E growth in cultures with 100 U/ml TNF, but had little effect on cultures containing 1000 U/ml of either IFN‐α or TNF. The aberrant in vitro response to IFN‐γ demonstrated in some of these patients may be of relevance to the pathophysiology of MPD. These results fail to demonstrate a differential in vitro effect for IFN‐α on MPD BFU‐E growth compared with controls and suggest that the in vitro suppression of haemopoiesis by IFN‐α when used in MPD treatment is non‐specific.