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Interleukin‐4 induces proliferation of adult T‐cell leukemia cells
Author(s) -
Mori Naoki,
Yamashita Uki,
Tanaka Yoshiya,
Nakata Koichi,
Oda Susumu,
Morimoto Isao,
Eto Sumiya
Publication year - 1993
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1993.tb00081.x
Subject(s) - autocrine signalling , paracrine signalling , t cell leukemia , cell growth , cytokine , flow cytometry , leukemia , biology , interleukin 2 , cancer research , interleukin 3 , cell culture , receptor , microbiology and biotechnology , immunology , t cell , interleukin 21 , immune system , biochemistry , genetics
Abstract: To evaluate the effect of IL‐4 on the growth of leukemic cells from adult T‐cell leukemia (ATL) patients (ATL cells) and determine whether the IL‐4 autocrine mechanism is involved in the growth of ATL cells, we studied the proliferative response of ATL cells, from 11 patients, cultured in the presence or absence of IL‐4 in vitro. Leukemic cells from 10 of the 11 patients examined proliferated in response to both IL‐2 and IL‐4 in a dose‐dependent manner. The proliferative response to IL‐4 was higher than that obtained with IL‐2 in 8 patients. The expression of the IL‐2 receptor (IL‐2R) αα‐chain in leukemic cells from some patients was also enhanced by IL‐4. The IL‐4 receptor was demonstrated by flow cytometry on the surface of ATL cells. Neither IL‐4‐induced proliferation of ATL cells nor IL‐4‐induced IL‐2R expression on ATL cells was inhibited by anti‐Tac or anti‐IL‐2 antibody and, therefore, these effects of IL‐4 are considered independent of endogenous IL‐2 activity. However, IL‐2 and IL‐4 were undetectable in the culture supernatants of ATL cells from any patient by enzyme‐linked immunosorbent assay. Interferon‐γ (IFN‐γ) partially inhibited IL‐2‐ or IL‐4‐induced proliferation of ATL cells. These results suggest that leukemic cells from ATL patients proliferate by an IL‐2 or IL‐4 paracrine mechanism in lymphoid tissue in vivo and that IFN‐γ inhibits IL‐2‐ or IL‐4‐induced proliferation of ATL cells.

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