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Misincorporation of uracil into the DNA of folate‐ and B 12 ‐deficient HL60 cells
Author(s) -
Wickramasinghe S. N.,
Fida S.
Publication year - 1993
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1993.tb00080.x
Subject(s) - uracil , hydrolysate , deoxyuridine , uridine , dna , microbiology and biotechnology , biochemistry , chemistry , nucleotide , l1210 cells , cell culture , thymidine , hydrolysis , biology , rna , cytotoxicity , in vitro , genetics , gene
HL60 cells were cultured for 10 days under various experimental conditions. They were then incubated with 1 μmol/l [5‐ 3 H] uridine for 2 hours and their DNA extracted. The DNA was hydrolysed to deoxyribonucleosides with phosphodiesterase and alkaline phosphatase and the hydrolysate subjected to Aminex A6 chromatography. The elution profiles showed that, when compared with control cells, DNA from cells grown in medium deficient in folate, B 12 or both folate and B 12 contained increased amounts of deoxyuridine (dU) and increased radioactivity in the dU peak. The data demonstrate that misincorporation of uracil into DNA occurs in a myeloid cell line cultured in growth medium deficient in folate, B 12 or both folate and B 12 .