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Red cell destruction by human monocytes — changes in intracellular ferritin concentration and phenotype
Author(s) -
RahaChowdhury R.,
Williams B. J.,
Worwood M.
Publication year - 1993
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1993.tb00070.x
Subject(s) - ferritin , incubation , peripheral blood mononuclear cell , chemistry , intracellular , endocrinology , medicine , monocyte , ferric , antibody , microbiology and biotechnology , andrology , immunology , biochemistry , biology , in vitro , organic chemistry
Mononuclear cells from 5 normal men and 5 patients homozygous for hereditary haemochromatosis (HFE) have been incubated for 18 h with or without the addition of sheep red blood cells coated with antibody (SRBC). In the absence of SRBC mean H type ferritin concentrations were greater than L type (normals: mean L type 11.6 ng/10 6 cells, H type 15.5; patients, L type 23.5 ng/10 6 cells, H type 41.6). In the presence of SRBC, monocyte L type ferritin concentrations increased considerably (76 ng/10 6 cells in normals and 141 ng/10 6 cells in patients) but H type ferritin concentrations were the same or decreased compared with incubation in medium only. Incubation with additional iron (ferric ammonium citrate, 2.5 μg Fe/ml) increased both H and L type ferritin concentrations. Erythrophagocytosis thus appears to cause differential regulation of H and L ferritin subunit synthesis or breakdown. Normal subjects and patients do not differ in this response to erythrophagocytosis.