Premium
Identification of a normal human bone marrow cell population co‐exmessin‐g megakaryocytic and erythroid markers in culture
Author(s) -
Bellucci Sylvia,
Han Zhong C.,
Pidard Dominique,
Caen Jacques Philippe
Publication year - 1992
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1992.tb01804.x
Subject(s) - megakaryocyte , haematopoiesis , bone marrow , biology , phenotype , population , glycophorin , lineage markers , cell culture , microbiology and biotechnology , immunology , pathology , antigen , stem cell , gene , genetics , medicine , environmental health
A population of haematopoietic cells co‐expressing glycoprotein IIIa (GPIIIa), which has been shown to be present in the megakaryocyte‐platelet lineage, and glycophorin A, which has been shown to be specific for the erythroid lineage, has been identified in normal bone marrow cultures using double immunofluorescence staining. The cells showing this phenotype have the size of lymphocytes and appear at an early time (day 1 to d 6) of culture. We have also detected a type of mixed megakaryocyte (MK) cluster containing this phenotype on d 3 or 4 in most normal marrow samples. Such a cell phenotype was not detected after 6 d of culture. It is thus the first time that this phenotype, although already described for several human neoplastic cell lines, has been observed in several normal human bone marrow cultures. Cells expressing this phenotype may represent haematopoietic cells, common to megakaryocytic and erythroid lineages, at a very early stage of differentiation.