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IL2‐ and IL4‐dependent proliferation of T‐cell clones derived early after allogeneic bone marrow transplantation: Studies of patients with chronic myelogenous leukaemia
Author(s) -
Bruserud Ø.,
Hamann W.,
Patel S.,
Ehninger G.,
Pawelec G.
Publication year - 1992
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1992.tb01589.x
Subject(s) - interleukin 4 , bone marrow , t cell , peripheral blood mononuclear cell , immunology , transplantation , biology , cell growth , interleukin 2 , cancer research , medicine , cytokine , in vitro , immune system , genetics
In an attempt to explore T‐cell functions shortly after allogeneic bone marrow transplantation more fully, IL2‐ and IL4‐dependent proliferation was assessed on CD4 + TCRαβ + T‐cell clones derived 4–6 weeks after transplantation. Both allogeneic pooled peripheral blood mononuclear cells and Epstein‐Barr virus‐transformed B‐cell lines (BCL) could function as accessory cells (AC) for PHA activation of T‐cell clones. Although minimal clonal proliferation was seen when the T‐cell activation signal was BCL + PHA + IL4, a majority of the clones could undergo IL4‐dependent proliferation after previous activation with AC + PHA + IL2. For certain clones, IL4 also showed an additive effect with IL2. Thus, IL4 was a growth factor for a majority of the investigated posttransplant T‐cell clones, and in vivo modulation of IL4‐dependent T‐cell functions may thus become a future therapeutic possibility to enhance graft‐versus‐leukaemia effects in bone marrow transplant recipients.