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Expression of adhesion antigens of human bone marrow megakaryocytes, circulating megakaryocytes and blood platelets
Author(s) -
Pampus Elisabeth C. M.,
Denkers Ingrid A. M.,
Geel Björn J. M.,
Huijgens Peter C.,
Zevenbergen Adri,
Ossenkoppele Gert J.,
Langenhuijsen Mart M. A. C.
Publication year - 1992
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1992.tb00915.x
Subject(s) - platelet , bone marrow , megakaryocyte , antigen , biology , pathology , immunology , immunohistochemistry , microbiology and biotechnology , medicine , haematopoiesis , stem cell
There is evidence that mature megakaryocytes migrate into sinusoids, enter the blood and fragment in the vascular bed. We wondered whether differences in expression of adhesion antigens could be associated with the egress of megakaryocytes from bone marrow into the peripheral blood or the fragmentation into platelets. Megakaryocytes from human marrow were purified by counterflow centrifugal elutriation followed by a glycoprotein Ib‐dependent agglutination procedure. Megakaryocytes from central venous blood and pulmonary arteries were purified by counterflow centrifugal elutriation alone. Adhesion antigens were labelled in an immunohistochemical assay. Both bone marrow megakaryocytes and platelets from healthy volunteers stained >75% positive for CD36, CD41, CD42, Cdw49b (α subunit VLA2), Cdw49e (α subunit VLA5), Cdw49f (α subunit VLA6) and CD62. Circulating megakaryocytes, although >75% positive for CD41, had, unlike platelets and bone marrow megakaryocytes, a reduced and remarkable heterogenous (5–100% positive) labelling with antibodies against Cdw49b, Cdw49e, Cdw49f. These results could be confirmed by comparing the bone marrow megakaryocytes, circulating megakaryocytes and platelets from 7 patients that were recovered and processed at the same time. Morphologically mature, circulating megakaryocytes have, unlike bone marrow megakaryocytes, a heterogenous expression of adhesion antigens, especially of Cdw49b, Cdw49e, and Cdw49f.