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Further studies of the defective stimulus‐response coupling for the oxidative burst in neutrophils in polycythemia vera
Author(s) -
Samuelsson Jan,
Berg Anders
Publication year - 1991
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1991.tb01565.x
Subject(s) - respiratory burst , polycythemia vera , chemotaxis , endocrinology , medicine , agonist , receptor , chemistry , n formylmethionine leucyl phenylalanine , immunology , biochemistry
Samuelsson J, Berg A. Further studies of the defective stimulus‐response coupling for the oxidative burst in neutrophils in polycythemia vera. Eur J Haematol 1991: 47: 239–245. Abstract: We have previously reported that the chemiluminescence (CL) response of neutrophils (PMN) from patients with polycythemia vera (PV) was abnormally low when induced by surface receptor‐dependent stimuli, fMLP and leukotriene B 4 , but normal when elicited by phorbol myristate acetate (PMA). This study documents that this discrepancy of the CL response to fMLP and PMA remained over a wide range of stimuli concentrations, was not due to iron‐deficient PV cells and was also observed with the nitroblue tetrazolium assay. Moreover, another surface receptor‐dependent agonist, platelet‐activating factor, conferred a significantly lower CL response in PV PMN relative to controls. Treatment with alpha interferon or GM‐CSF, to increase fMLP receptors, resulted in a similar enhancement of fMLP‐induced CL in PV and controls. CL was normal when induced by a number of non‐surface receptor‐dependent stimuli. Release of lactoferrin in response to fMLP (and PMA) was normal (as was previously reported fMLP‐induced chemotaxis and adherence). Thus, this defect is highly specific for oxidative metabolism, and localized to discrete step(s) of the stimulus‐response coupling for fMLP, leukotriene B 4 and PAF, but conceivably not due to impairment of the dynamic interaction of fMLP with its receptor.