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Peripheral blood T lymphocytes in patients with monoclonal gammopathies: Expanded subsets as depicted by capacity to bind to autologous monoclonal immunoglobulins or reactivity with anti‐V gene‐restricted antibodies
Author(s) -
Österborg A.,
Janson C. H.,
Bergenbrant S.,
Holm G.,
Lefvert AK,
Wigzell H.,
Mellstedt H.
Publication year - 1991
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1991.tb01553.x
Subject(s) - idiotype , monoclonal antibody , clone (java method) , monoclonal , cd8 , antibody , t cell receptor , t cell , immunoglobulin idiotypes , immunology , microbiology and biotechnology , cytotoxic t cell , biology , antigen , gene , in vitro , immune system , genetics
Abstract: The presence of T cells binding F(ab‘) 2 fragments of the idiotypic immunoglobulin was examined by immunofluorescence in peripheral blood of patients with monoclonal gammopathy. In 3 out of 11 tested patients, 1–15% idiotype‐binding T cells of either CD4 or CD8 phenotype were found. In 1 patient both a CD4 + and a CD8 + idiotype‐binding T‐cell fraction were present. In 1 patient the idiotype‐binding T cells also reacted with a mAb directed against the variable parts of the TCR α or β chains, further indicating a clonal origin at the α/β level. 3 patients had an expanded predominant T‐cell receptor V gene usage based on the reactivity with the limited panel of TCR mAb, but these “clonal” T cells did not bind the idiotype. The study supports the existence of idiotype‐specific T cells in peripheral blood of patients with monoclonal gammopathy. Such cells might have a regulatory role on the monoclonal B‐cell clone and may be an important target for immunotherapy.