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Cell cycle arrest and DNA damage after melphalan treatment of the human myeloma cell line RPMI 8226
Author(s) -
Fernberg JanOlof,
Lewensohn Rolf,
Skog Sven
Publication year - 1991
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1991.tb01549.x
Subject(s) - melphalan , dna , dna damage , cell cycle , cell culture , microbiology and biotechnology , chemistry , cell , multiple myeloma , biology , biochemistry , genetics , immunology
Exposure of a myeloma cell line (RPMI 8226) to a 30‐minute pulse of melphalan (1‐phenylalanine‐mustard) resulted in a cell cycle progression delay characteristic for DNA cross‐linking agents. Reduction of outflow of cells from late S‐ and G 2 ‐phases was more pronounced as compared to that from G 1 ‐phase. The consequence is a progressive accumulation of cells in late S‐ and G 2 ‐phases. At restoration of outflow of cells from late S‐ and G 2 ‐phases, complete removal of DNA interstrand cross‐links, as measured by DNA alkaline elution, was noted. At this time less than 50% of maximum DNA‐protein cross‐links were removed. Further we found no correlation between restored outflow of cells from the G 2 ‐phase and removal of DNA‐protein cross‐links during the follow‐up time of 72 h. No DNA double strand breaks as measured by DNA neutral elution were formed during the observation period. The data suggest that removal of DNA interstrand cross‐links seems prerequisite for the outflow of cells from G 2 after melphalan treatment.