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Regeneration of CALLA (CD10 + ), TdT + and double‐positive cells in the bone marrow and blood after autologous bone marrow transplantation
Author(s) -
Smedmyr Bengt,
Bengtsson Mats,
Jakobsson Åke,
Simonsson Bengt,
Öberg Gunnar,
Tötterman Thomas H.
Publication year - 1991
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1991.tb01268.x
Subject(s) - calla , bone marrow , medicine , peripheral blood mononuclear cell , pathology , immunology , biology , antibody , in vitro , monoclonal antibody , biochemistry
In this prospective study we investigated the frequency of CD10 + , TdT + and CD10 + TdT + mononuclear cells in the bone marrow (BM) and peripheral blood (PB) before and after autologous bone marrow transplantation (ABMT). 49 patients treated for acute lymphoblastic or myeloblastic leukaemia, malignant lymphoma or multiple myeloma were included. A significant increase in CD10 + cells occurred in BM in both children and adults after ABMT. In children, we also found a significant increase in CD10 + cells in PB. In individual patients remaining in remission, up to 34% CD10 + cells having a normal Ig kappa/lambda light chain ratio were recorded after ABMT. In children, the percentage of TdT + and CD10 + TdT + cells increased significantly in BM. In most cases the CD10/TdT‐ratio was greater than 1.0, but during early regeneration after ABMT this ratio was less than 1.0 in several patients remaining in complete remission. In patients remaining in remission, CD10 + TdT + cells were detected in the blood in only 2 out of 140 samples tested, and the proportion of these cells never exceeded 0.03%. We conclude that quantitation of CD10 + TdT + cells in peripheral blood is helpful in the evaluation of complete remission in patients treated for pre‐B‐ALL.