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Distinct morphophenotypic features of chronic B‐cell leukaemias identified with CDlc and CD23 antibodies
Author(s) -
Orazi Attilio,
Cattoretti Giorgio,
Polli Nicoletta,
Delia Domenico,
Rilke Franco
Publication year - 1991
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1991.tb00557.x
Subject(s) - cd23 , b cell , chronic lymphocytic leukemia , autoanalyzer , medicine , pathology , cd38 , immunology , bone marrow , monoclonal antibody , antibody , spleen , monoclonal , lymphoproliferative disorders , lymphoma , leukemia , biology , immunoglobulin e , stem cell , cd34 , genetics
Morphological criteria usually applied to diagnose various subtypes of B‐cell chronic lymphoid leukaemia are largely subjective. Immu‐nophenotyping of 61 relevant cases using a selected panel of monoclonal antibodies (mAb), showed that CDlc and CD23 mAb were able to separate B‐cell chronic lymphocytic leukaemia (B‐CLL) from other chronic B‐cell lymphoproliferative diseases. Lymphocytes of B‐CLL were CDlc ‐ , CD23+, whereas those of other types of chronic B‐cell leukaemia were CDlc +/‐ , CD23 ‐ , and CD38 +/‐ . Non‐B‐CLL cases had a significantly higher amount of large peroxidase‐negative (unstained) cells analyzed with an automated blood cell counter (Technicon H6000). This type of volumetric assessment allowed a separation between typical and “atypical” B‐CLL, which otherwise were both CDlc ‐ , and CD23+. These combinations of phenotypic markers corresponded to well‐defined haematopathologic entities, conventionally diagnosed on peripheral blood (PB) and bone marrow smears, and on histologic sections of lymph nodes and spleen.