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Regulation of mRNA levels of alkaline phosphatase gene in neutrophilic granulocytes by granulocyte colony‐stimulating factor and retinoic acid
Author(s) -
Sato Noriharu,
Mizukami Hiroaki,
Tani Kenzaburo,
Asano Shigetaka
Publication year - 1991
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1991.tb00530.x
Subject(s) - retinoic acid , granulocyte , granulocyte colony stimulating factor , alkaline phosphatase , messenger rna , gene , acid phosphatase , microbiology and biotechnology , chemistry , biology , immunology , biochemistry , enzyme , genetics , chemotherapy
We examined steady‐state levels of mRNA for alkaline phosphatase in neutrophils (NAP) treated with granulocyte colony‐stimulating factor (G‐CSF). The amount of mRNA for NAP was shown to increase after 6 hours of culture with G‐CSF when no increase in NAP activity was yet observed, and the transcript was the greatest after 20–24 h of culture with G‐CSF. Treatment of neutrophils with both G‐CSF and retinoic acid augmented the amount of mRNA for NAP over the amount obtained by G‐CSF alone, which was most marked at 24 h. These results show that both G‐CSF‐mediated NAP induction and its potentiation by retinoic acid are due to the increased levels of mRNA for NAP.