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Influences of antiplatelet autoantibodies on platelet function in immune thrombocytopenic purpura
Author(s) -
Yanabu Mutsumasa,
Suzuki Masahiko,
Soga Tetsuji,
Sone Naoaki,
Nagata Hirokazu,
Nomura Shosaku,
Kokawa Terutoshi,
Yasunaga Kojiro
Publication year - 1991
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1991.tb00529.x
Subject(s) - autoantibody , platelet , platelet membrane glycoprotein , ristocetin , antibody , immunology , monoclonal antibody , platelet glycoprotein gpib ix complex , medicine , flow cytometry , immune system , glycoprotein ib , thrombocytopenic purpura , von willebrand factor
We investigated the characteristics of the antiplatelet autoantibodies in 60 patients with ITP. Using flow cytometry, the binding of monoclonal antibodies to the platelet glycoprotein (GP) IIb/IIIa complex and to GPIb was examined in these patients. The extent of binding was decreased in 15 patients (anti‐GPIIb/IIIa in 12 patients and both anti‐GPIIb/IIIa and anti‐GPIb in 3 patients). Western blotting revealed that 10 of these 15 patients had either anti‐GPIIb or anti‐GPIIIa and 2 had anti‐GPIb autoantibodies. ADP‐induced aggregation of normal platelets was inhibited by autoantibodies in 12 of 60 patients, and 11 of these had anti‐GPIIb/IIIa antibodies. Ristocetin‐induced aggregation was inhibited in 4 of these patients, and 2 with prominent inhibition had anti‐GPIb antibodies. There was a significant relationship between platelet‐associated IgG value and ATP secretion. These results suggest that some antiplatelet autoantibodies can affect platelet function and thus have an influence on the pathophysiology of ITP.