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Regulation of autologous T‐lymphocyte clones on in vitro growth of BFU‐e: Enhancing effects of both CD4‐ and CD8‐positive clones
Author(s) -
Nielsen B.,
Jessen P.,
Hokland P.
Publication year - 1991
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1991.tb00509.x
Subject(s) - cd8 , t lymphocyte , in vitro , biology , immunology , lymphocyte , microbiology and biotechnology , phenotype , major histocompatibility complex , cytotoxic t cell , immune system , genetics , gene
In order to characterize the effect of T lymphocytes on the in vitro growth of erythroid progenitor cells (EPC), we have optimized an assay for evaluating the influence of autologous T‐ lymphocyte clones on BFU‐e, grown from PBMNCs depleted of T lymphocytes. Testing a large number of T‐lymphocyte clones from 2 individuals we found that, irrespective of immunological phenotype, all clones tested were found to enhance the growth of BFU‐e. Thus, no evidence for a functional dichotomy between CD4+ and CD8+ T lymphocytes was found in this system. The enhancing effect worked across a histocompatibility barrier and was, at least in part, mediated by soluble factors. We therefore conclude that the role of the majority of cloned normal T lymphocytes in the regulation of the in vitro growth of EPC is a supportive one.