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Constitutive production of granulocyte colony‐stimulating factor and interleukin‐6 by a human lung cancer cell line, KSNY: Gene amplification and increased mRNA stability
Author(s) -
Matsuguchi Tetsuya,
Okamura Seiichi,
Kawasaki Chiyuki,
Shimoda Kazuya,
Omori Fusayuki,
Hayashi Shin,
Kimura Nobuhiro,
Niho Yoshiyuki
Publication year - 1991
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1991.tb00135.x
Subject(s) - cell culture , granulocyte , microbiology and biotechnology , biology , granulocyte colony stimulating factor , northern blot , neutrophilia , messenger rna , gene , cytokine , interleukin , cancer research , gene expression , immunology , genetics , chemotherapy
A human lung squamous cell carcinoma cell line designated KSNY was established from a patient suffering from marked neutrophilia and polyclonal hyper‐γ‐globulinemia. In our previous report, we demonstrated colony‐stimulating activities in the culture supernatant of this cell line. To determine the exact molecules for the activities, we investigated the gene expression of various cytokines in KSNY cells and showed the mRNA expression of both granulocyte colony‐stimulating factor (G‐CSF) and interleukin‐6 (IL‐6). We also detected substantial amounts of G‐CSF and IL‐6 in the culture supernatant with sensitive enzyme‐linked immunosorbent assays (ELISA). The amplification of the gene locus for G‐CSF, but not for IL‐6 was shown by Southern blot analysis. Furthermore, we also showed that the mRNAs for G‐CSF and IL‐6 were relatively stable in KSNY cells. These findings are thought to be related to the constitutive production of the cytokines in KSNY cells.