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Labelling autologous platelets with 111 In tropolonate for platelet kinetic studies: limitations imposed by thrombocytopenia
Author(s) -
Danpure H. J.,
Osman S.,
Peters A. M.
Publication year - 1990
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1990.tb00461.x
Subject(s) - platelet , labelling , kinetic energy , medicine , chemistry , immunology , biochemistry , physics , quantum mechanics
An in vitro method of radiolabelling platelets with 111 In tropolonate in plasma has been devised enabling imaging and cell kinetic studies to be performed in patients with thrombocytopenia (TP) using autologous, rather than donor, platelets. Platelets from 10 TP patients, with platelet counts ranging from 4–91 times 10 9 /1, were labelled in 50% plasma with 111 In tropolonate, containing the optimum tropolone concentration of 2 times 10 −4 mol/l, at platelet concentrations ranging from 0.08‐4.5 times 10 9 /ml resulting in labelling efficiencies (LE) between 51 and 86%. In 4 patients, red cells contaminated the platelets but this was corrected for by measuring the proportion of 111 In on the platelets prior to injection and in the post‐injection blood samples. Platelet recovery (PR), mean platelet life‐span (MPLS) and platelet production rate (PPR) were calculated and splenic and hepatic images were taken. The results clearly show that useful clinical data can be obtained by this method even in patients with severe TP.