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Immunogenotyping with antigen receptor gene probes as a diagnostic tool in childhood acute lymphoblastic leukaemia
Author(s) -
Fey M. F.,
Tobler A.,
Stadelmann B.,
Hirt A.,
Theilkäs L.,
Khandjian E. W.,
RidolfiLüthy A.,
Delaleu B.,
Weil R.,
Wagner H. P.
Publication year - 1990
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1990.tb00460.x
Subject(s) - medicine , antigen , immunology , pathology
13 cases of childhood acute lymphoblastic leukaemia (ALL) were studied combining cell surface marker analysis with immunogenotyping by Southern blot hybridisation with a panel of antigen receptor gene probes. The immunophenotypes were unequivocal: 7 patients had B‐phenotype and 6 patients T‐phenotype ALL. In several patients immunogenotypes were not fully consistent with the respective phenotypes. For example, 2 B‐cell precursor ALL had rearranged TCRβ chain genes and 2 T‐ALL rearrangement of Ig heavy‐chain genes. All cases showed clonal rearrangement or deletions within the TCRδ gene locus. TCRδ gene rearrangements might, therefore, serve as markers of clonality but not of B‐ or T‐lineage in immature lymphoid neoplasms. We conclude that in current diagnostic practice immunogenotyping is a supplement rather than an alternative to immunophenotyping by surface marker analysis.