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Allo‐activated CD4+ and CD8+ lymphocyte subsets: New ultrastructural findings based on computer‐assisted image analysis
Author(s) -
Balercia G.,
Sbarbati A.,
Franceschini F.,
BravoCuellar A.,
Osculati A.,
Accordini C.,
OrbachArbouys S.
Publication year - 1990
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1990.tb00373.x
Subject(s) - organelle , cytoplasm , cd8 , microbiology and biotechnology , stimulation , biology , ultrastructure , lymphocyte , t cell , lymphocyte subsets , immunology , immune system , neuroscience , anatomy
Primary allo‐stimulation induces substantial and differentiated modifications in the development of cytoplasmic organelles of peripheral T‐lymphocyte subsets. The present computer‐assisted image analysis provides evidence that the CD4 + subset undergoes significant increase in organelle density after stimulation, while the CD8 + subset exhibits a post‐stimulation organelle development proportional to the cell size. Each immunological subset contains two distinct patterns of cytoplasmic organization, here defined as type I (containing a few cytoplasmic organelles) and type II (with well‐developed synthesis and storage organelles). On the basis of these findings cytologic parameters could be ascribed to a heterogeneous proliferation of functionally distinct T‐cell clones in CD4 + and CD8 + subsets.