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Synthesis of a 60 kD nuclear DNA binding protein induced by cytosine arabinoside in the HL 60 leukemic cell line
Author(s) -
Ravazzolo Roberto,
BianchiScarrǎ Giovanna,
Capra Valeria,
Fiorentini Paola,
Garrě Cecilia
Publication year - 1990
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1990.tb00368.x
Subject(s) - cytosine , dna , methionine , cell culture , cytotoxic t cell , nuclear protein , microbiology and biotechnology , chemistry , dna synthesis , biochemistry , cancer research , biology , amino acid , gene , genetics , in vitro , transcription factor
Cytosine arabinoside (ara‐C) is being employed at low dosage as differentiative rather than a cytotoxic agent in the therapy of leukemias. We have analyzed nuclear proteins from HL 60 leukemic cells treated with ara‐C and have observed increased expression of a 60 kD protein in a dose‐dependent fashion. This protein is actively synthesized, as assessed by labeled methionine incorporation. Using DNA cellulose affinity chromatography we could also demonstrate DNA binding properties of the 60 kD protein.