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Ferritin synthesis in peripheral blood lymphocytes following in vitro stimulation*
Author(s) -
Pattanapanyasat Kovit
Publication year - 1989
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1989.tb01470.x
Subject(s) - phytohaemagglutinin , ferritin , in vitro , stimulation , intracellular , incubation , dna synthesis , immunoprecipitation , chemistry , endocrinology , peripheral blood , biology , biochemistry , medicine , microbiology and biotechnology , immunology , antibody
The incubation of normal peripheral blood lymphocytes with phytohaemagglutinin results in an increase in DNA synthesis accompanied by an increase in ferritin synthesis, as determined by 3 H‐leucine incorporation into ferritin isolated by immunoprecipitation. The increase was 5 to 8 times greater than that of non‐stimulated cells. This phenomenon was associated with a 3‐ to 6‐fold increase of intracellular ferritin when compared with the original ferritin concentration. While the significance of increased ferritin expression in proliferative cells is not known, an increase in ferritin synthesis following in vitro stimulation is probably related to increased metabolic activity of the cells.