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Some methods and applications of flow cytometric DNA analysis in clinical and experimental oncology
Author(s) -
Vindeløv Lars L.,
Christensen Ib J.
Publication year - 1989
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1989.tb01240.x
Subject(s) - standardization , set (abstract data type) , medical physics , computer science , oncology , computational biology , dna , sample (material) , medicine , pathology , biology , chromatography , genetics , chemistry , programming language , operating system
An integrated set of methods for routine flow cytometric DNA analysis are described. The methods consist of procedures for sample acquisition, storage, standardization, staining and statistical analysis. Our experience with the methods over the past ten years is reviewed. It is concluded that only with adequate solutions to all methodological steps involved, is it possible to produce reliable end‐point results. Flow cytometric DNA analysis is a valuable research tool in clinical and experimental oncology. Final evaluation of the prognostic significance of DNA analysis in neoplastic disease awaits the results of ongoing studies.