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Binding patterns of monoclonal anti‐B, anti‐H and anti‐(Le b + Y) on erythrocytes, imaged in the scanning electron microscope
Author(s) -
Heier Hans Erik,
Namork Ellen
Publication year - 1989
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1989.tb00287.x
Subject(s) - immunogold labelling , antigen , monoclonal antibody , electron microscope , scanning electron microscope , chemistry , microbiology and biotechnology , glycolipid , antibody , biology , crystallography , biochemistry , immunology , physics , optics
Binding patterns of monoclonal IgM anti‐B, anti‐H and anti‐(Le b + Y) antibodies on erythrocytes were visualized in the backscatter electron imaging (BEI) mode of a scanning electron microscope by indirect immunogold labelling. The results were independent of secretor status, supporting the view that type 2 oligosaccharides predominate on erythrocytes. There were considerable cell‐to‐cell variations of amounts of the antigens, presumably because of differences of antigen development. The amount of H antigens may have been overestimated in previous studies. B and H antigens seemed linked mostly to mobile structures, probably lipid, on the convex parts of the cells and to immobile structures, probably protein, on the concave parts. Y antigens appeared bound to mobile structures also on the concave part of the cell membrane. The results therefore confirm that B oligosaccharides on erythrocytes develop from both lipid‐ and protein‐bound H, but suggest that Y develops mainly from lipid‐bound H.