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Molecular analysis of the bcr rearrangement in a case of Ph‘‐negative blastic crisis of Ph’‐positive chronic myelogenous leukemia
Author(s) -
Melani Cecilia,
Canepa Letizia,
Sessarego Mario,
Miglino Maurizio,
Ferraris Anna Maria,
Gaetani Gian Franco
Publication year - 1989
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1989.tb00243.x
Subject(s) - chronic myelogenous leukemia , blast crisis , breakpoint cluster region , leukemia , medicine , cancer research , philadelphia chromosome , chemistry , chromosomal translocation , biochemistry , receptor , gene
We describe here a patient with Ph'‐positive chronic myelogenous leukemia (CML) who developed a Ph'‐negative blastic crisis. The blast DNA was analyzed on two different occasions, at the beginning of the blastic phase and at the end, shortly before the patient's death. Although cells from both samples had no Ph' chromosome marker (not even a masked one) we could detect a rearrangement of the bcr gene in the second DNA sample, using a ‘3′‐bcr’ probe. The same probe and a ‘5′‐bcr’ probe failed to detect any rearranged band in the first DNA sample. No rearrangement was identified at the c‐myc and N‐ras loci, while a slight c‐myc amplification was evident in both DNA samples tested.