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Serum from patients with various thrombopoietic disorders alters terminal cytoplasmic maturation of human megakaryocytes in vitro
Author(s) -
Straneva John E.,
Briddell Robert A.,
Hui Siu L.,
Hoffman Ronald
Publication year - 1989
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1989.tb00115.x
Subject(s) - megakaryocytopoiesis , thrombocytosis , haematopoiesis , biology , aplastic anemia , platelet , cytoplasm , immunology , in vitro , progenitor cell , wiskott–aldrich syndrome , microbiology and biotechnology , megakaryocyte , endoreduplication , incubation , andrology , ploidy , bone marrow , stem cell , medicine , genetics , biochemistry , gene
Human bone marrow was depleted of progenitors (CFU‐MK), but enriched for recognizable megakaryocytes (MK), and placed in cultures with serum from either normal donors (NABS) or patients with primary (PTS) or secondary (STS) thrombocytosis, autoimmune thrombocytopenia (ATS) or aplastic anemia (AAS). Mean MK diameters shifted during the 3–4 days of incubation. Endomitotic figures were visible and mean ploidy increased slightly during cytoplasmic maturation, where decreases in immature cells (stages 1 and 2) were accompanied by increases in the mature MK (stages 3 and 4). Cytoplasmic maturation was faster in AAS, ATS and STS than PTS or NABS; mean size and ploidy were similar in all cultures. Recognizable MK were not forced to undergo additional endoreduplication in response to stimulation. Only AAS augmented MK colony formation, which indicated that at least two humoral factors can regulate megakaryocytopoiesis at separate levels, the progenitors and morphologically recognizable MK.