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Immunotoxins containing saporin 6 and monoclonal antibodies recognizing plasma cell‐associated antigens: Effects on target cells and on normal myeloid precursors (CFU‐GM)
Author(s) -
Barbieri Luigi,
Dinota Angelo,
Gobbi Marco,
Tazzari Pier Luigi,
Rizzi Simonetta,
Bontadini Andrea,
Lemoli Roberto Massimo,
Tura Sante,
Stirpe Florenzo
Publication year - 1989
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1989.tb00105.x
Subject(s) - immunotoxin , saporin , ribosome inactivating protein , monoclonal antibody , antibody , microbiology and biotechnology , ricin , k562 cells , antigen , cancer research , cell culture , myeloid , biology , chemistry , immunology , leukemia , biochemistry , toxin , genetics , ribosome , rna , gene
Monoclonal antibodies 8A and 62B1, recognizing plasma cell‐associated antigens, were covalently linked to saporin 6, a ribosome‐inactivating protein similar to the A‐chain of ricin. Both immunotoxins were tested on target human cell lines U266 and Raji, on non‐target K562 cell line and on myeloid CFU‐GM progenitors. The cloning efficiency and viability of target cells were strongly reduced by 8A‐saporin 6 and 62B1‐saporin 6 immunotoxins, with an ID 50 up to 2‐fold lower than free saporin 6, whilst the K562 non‐target cell line was unaffected. Normal human myeloid precursors (CFU‐GM) were inhibited by immunotoxins only to a limited extent. An application of this model for autologous bone marrow transplantation in multiple myeloma patients is proposed. Since no eradication of cloning target cells was achieved by a single immunotoxin, mixtures made with different antibodies could help to reach this goal.