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Lactoferrin‐mediated transfer of iron to intracellular ferritin in human monocytes
Author(s) -
Birgens H. S.,
Kristensen L. Østergaard,
Borregaard N.,
Karle H.,
Hansen N. E.
Publication year - 1988
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1988.tb00868.x
Subject(s) - lactoferrin , ferritin , cytosol , intracellular , incubation , chemistry , monocyte , macrophage , transferrin , size exclusion chromatography , biochemistry , biology , immunology , in vitro , enzyme
Interactions of 125 I‐ 59 Fe‐lactoferrin with human monocytes were studied. After 4 hours of incubation, the uptake of 59 Fe exceeded that of 125 I. In dissociation studies the cellular 59 Fe‐activity was only partly dissociable during 16 h, whereas the 125 I‐activity could be released nearly completely. Disruption of the cells and studies on the cytosolic phase were performed employing gel‐filtration and affinity chromatography. An appreciable amount of lactoferrin was found in the cytosolic phase. About 50% of the cytosolic 59 Fe‐activity was bound to ferritin. The results suggest that lactoferrin is internalized into monocytes and that iron is transferred to ferritin. These cellular events may contribute to an understanding of the accumulation of iron in the monocyte/macrophage system observed during inflammatory conditions.