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Kinetics of hemopoietic stem cells in a hypoxic culture
Author(s) -
Ishikawa Y.,
Ito T.
Publication year - 1988
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1988.tb00808.x
Subject(s) - haematopoiesis , progenitor cell , oxygen tension , bone marrow , in vitro , stem cell , mast cell , immunology , biology , chemistry , microbiology and biotechnology , andrology , oxygen , biochemistry , medicine , organic chemistry
The influence of low oxygen tension on the clonal growth of hemopoietic stem cells in vitro was examined. The numbers of colonies of neutrophil, macrophage, and eosinophil progenitors (CFU‐C), derived from human bone marrow, increased at a rate 1.7 times higher in low oxygen tension (7% O 2 ) than in a gas phase that contained air (19% O 2 ). The erythroid (BFU‐E) and multipotential (CFU‐mix) progenitors increased about 2.4 times in 7% O 2 , and the increase in the composed cell type of mixed colonies showed no rate difference in either gas phase. Under atmospheric conditions, a mouse mast cell progenitor (CFU‐mast) formed colonies, with the addition of 2‐mercaptoethanol (2‐ME). Under low oxygen tension, the CFU‐mast formed colonies without 2‐ME, but a further enhancement was observed with the addition of 2‐ME. Blood gas analysis of human bone marrow showed a pO 2 of 51.8 ± 14.5 mmHg, which was closed to O 2 tension in a gas phase culture media containing 7% O 2 . This data shows that the physiological O 2 tension enhances hemopoietic stem cell proliferation in vitro, and that a part of the enhancing effect by 2‐ME is due to a prevention of O 2 toxicity at 19% O 2 .