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Induction of human basophil histamine release by a novel protein kinase C activator, sn‐1,2‐isopropylidene‐3‐decanoyl‐glycerol (IpOCOC 9 ): Partial characterization of secretagogue characteristics
Author(s) -
Bergstrand Håkan,
Lundquist Britta,
Michelsen Peter
Publication year - 1988
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1988.tb00229.x
Subject(s) - histamine , secretagogue , chemistry , protein kinase c , extracellular , stimulation , endocrinology , medicine , biochemistry , receptor , kinase , biology
Human leukocytes were found to release histamine at exposure for the synthetic glyceride derivative sn‐1,2‐isopropylidene‐3‐decanoyl‐glycerol (IpOCOC 9 ). The following characteristics for the IpOCOC 9 ‐induced basophil histamine release were recorded. A. In the order of 25% of the cellular histamine content was extruded at 206 μmol/l and 45% at 690 μmol/l of the compound, respectively. B. Removal of extracellular Ca 2+ variably affected IpOCOC 9 ‐triggered release. C. The presence of N‐ethylmaleimide (10 μmol/l) or p‐bromophenacylbromide (10 μmol/l) markedly reduced IpOCOC 9 ‐induced histamine release. D. The time course of the release triggered by IpOCOC 9 was intermediate to those characterizing the release triggered by 4β‐phorbol 12‐myristate 13‐acetate (PMA) and by formyl‐methionyl‐leucyl‐phenylalanine (FMLP). E. Cells desensitized to IgE‐receptor‐mediated stimulation were hyperresponsive to stimulation with IpOCOC 9 . F. Cells treated with a low concentration of 2‐deoxyglucose were not hyperresponsive to IpOCOC 9 . These data show that IpOCOC 9 , a PMN/leukocyte protein kinase C stimulator, acts as a non‐cytotoxic secretagogue for human basophils with a mode of action which in some, but not all respects, mimics that of PMA. In particular, IpOCOC 9 ‐triggered release resembles that reported by other authors for hyperosmolar triggering of release by mannitol.