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Resistance of some leukemic blasts to lysis by lymphokine activated killer (LAK) cells
Author(s) -
Panayotides P.,
Porwit A.,
Sjögren AM,
Wasserman J.,
Reizenstein P.
Publication year - 1988
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1988.tb00192.x
Subject(s) - lymphokine activated killer cell , peripheral blood mononuclear cell , lymphokine , cytotoxic t cell , cytotoxicity , interleukin 2 , immunology , microbiology and biotechnology , natural killer cell , k562 cells , leukemia , chemistry , in vitro , medicine , biology , cytokine , interleukin 12 , immune system , biochemistry
Peripheral blood mononuclear cells (PBMC) from healthy donors and AML patients in remission were stimulated with phytohemagglutinin (PHA) and recombinant inter‐leukin‐2 (IL‐2). These stimulated cells (lymphokine activated killer (LAK) cells) showed increased DNA synthesis as measured by 3 H‐Thymidine uptake. A synergistic effect of PHA and IL‐2 was found. LAK cells' ability to kill acute myeloid leukemia (AML) blasts was investigated by the 51 Cr release assay. LAK cells showed a cytotoxicity (over 10% specific 51 Cr release) against 9/12 leukemic blasts, even at effector/target (E/T) ratios as low as 5:1. However, on average only 22.2% (SD 11.8) and 36.5% (SD 12.5) 51 Cr release were obtained in 4‐ and 18‐hour cytotoxicity assays, respectively, at an E/T ratio of 20:1. Leukemic blasts in 3/12 AML cases and normal PBMC were entirely resistant to lysis, even at an E/T ratio of 80:1. Susceptibility to lysis was not correlated to peanut‐agglutinin receptor expression. LAK cells were more cytotoxic towards the K‐562 cell line (natural killer activity) than unstimulated PBMC.