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A method combining morphological, immunocytochemical and chromosomal examinations of the same cell in the study of lymphoproliferative diseases
Author(s) -
Franssila K. O.,
Lindholm C.,
Teerenhovi L.,
Knuutila S.
Publication year - 1988
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1988.tb00187.x
Subject(s) - karyotype , clone (java method) , biology , pathology , lymphoproliferative disorders , population , mitosis , monoclonal , lymphoma , metaphase , chromosome , monoclonal antibody , immunology , antibody , medicine , genetics , dna , environmental health , gene
6 cases of different lymphoproliferative diseases were studied with the new MAC (Morphology‐Antibody‐Chromosome) method in order to find out 1) if the abnormal karyotype is confined to the monoclonal cell population, 2) if there are, within this clone, also cells with a normal karyotype, and 3) if the method can help the pathologist to diagnose malignant lymphoproliferative diseases. The MAC method allows a simultaneous study in the same metaphase cell of the karyotype, surface markers, and some morphological features. In all cases in which a monoclonal cell proliferation was detected immunohistologically, the MAC examination showed a chromosomal abnormality for the same light chain as was detected in immunohistology, but not in other cells. In all but a single case, all mitotic cells belonging to the clonal cell proliferation had an abnormal karyotype. In this case with lambda clonality, 2/8 lambda‐positive mitoses had a normal karyotype. However, all the normal mitoses occurred in small lymphocytes whereas the abnormal mitoses were seen in large blastic cells. In 1 case, the MAC method helped in confirming the diagnosis of malignant lymphoma (nodular small cleaved cell type). Especially in lymphomas composed of a mixed cell population, the MAC method makes it possible to find out which cell types have an abnormal karyotype and which have a normal karyotype.