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In vitro and in vivo behavior of 111 In‐labelled platelets: An experimental study of healthy male volunteers
Author(s) -
Wadenvik Hans,
Jacobsson Stefan,
Kutti Jack,
Syrjälä Martti
Publication year - 1987
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1987.tb01438.x
Subject(s) - platelet , mean platelet volume , whole blood , chemistry , spleen , in vivo , blood volume , labelling , isotopes of chromium , blood flow , in vitro , nuclear medicine , medicine , biochemistry , biology , microbiology and biotechnology , nuclear chemistry
The aim of this study was to obtain a critical evaluation of a simple method for labelling platelets with 111 In‐oxine. All experiments were carried out on healthy volunteers. 65 ± 7 (SD) % of the platelets in collected blood were labelled and reinjected. As compared to control experiments, only in response to a low final ADP concentration (1.0 μmol/l) did 111 In‐labelled platelets show reduced in vitro aggregability. The mean platelet volume for 111 In‐labelled platelets was slightly lower than the mean platelet volume in whole blood. The results for initial platelet recovery and platelet mean lifespan closely agreed with those of other studies in which considerably higher platelet extraction from whole blood was obtained. After injection, the splenic uptake and blood disappearance of 111 In‐labelled platelets followed a monoexponential function with almost identical rate constants. By compartmental analysis of the equilibration of platelets between blood and spleen, the splenic blood flow was estimated to be 4.8 ± 1.9 (SD) % of the total blood volume/min; the intrasplenic platelet transit time was 9.7 ± 1.6 (SD) min, and the exchangeable splenic platelet pool 31 ± 8 (SD) %. Highly significant relationships were present between the splenic blood flow and the splenic platelet pool size, as well as between the splenic blood flow and the initial platelet recovery. It is concluded that the requirements for adequate interpretation of platelet kinetics are well met with the present method for harvesting and labelling of platelets.