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Myelo‐monocytic differentiation potential of the CALLA‐positive Nairn 6M1 cell line
Author(s) -
Lau B.,
Jäger G.,
Lippl U.
Publication year - 1987
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1987.tb01155.x
Subject(s) - calla , monocyte , granulocyte , monoclonal antibody , precursor cell , cell culture , immunology , biology , microbiology and biotechnology , antibody , cell , biochemistry , genetics
Nairn 6M1 cells, derived from an acute lymphoblastic leukemia of the non‐T, non‐B type, are known to possess characteristics of early B‐lymphocytes. In order to determine whether these cells are able to differentiate along the granulocyte‐monocyte pathway, they were cultured in diffusion chambers, and classified according to morphological, cytochemical and immunological criteria. On the basis of this characterization, Nairn 6M1 cells positively labeling with monoclonal anti‐human granulocyte or monocyte‐specific antibodies were observed in all cases. These immunological changes were accompanied by the appearance of cells positive for the naphthyl‐AS‐D‐chloroacetate esterase reaction. However, no changes in the blast‐like appearance of the cultured cells were seen. From these data, we conclude that Nairn 6M1 cells already committed to the B‐cell lineage retain their capacity to develop cellular markers of the granulocyte‐monocyte series.