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Cytogenetic and immunologic characterization of mitotic cells in chronic lymphocytic leukaemia
Author(s) -
Autio K.,
Elonen E.,
Teerenhovi L.,
Knuutila S.
Publication year - 1987
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1987.tb00772.x
Subject(s) - karyotype , mitosis , biology , chronic lymphocytic leukemia , chromosome , cytogenetics , b cell , immunology , pathology , microbiology and biotechnology , leukemia , antibody , genetics , medicine , gene
Lymphocytes from 14 patients with chronic B‐cell leukaemia (B‐CLL) and one with chronic T‐cell leukaemia (T‐CLL) were studied by the MAC (Morphology, Antibodies, Chromosomes) method, which allows simultaneous analysis of the morphology, immunologic phenotype and karyotype of the same mitotic cell. Use of the MAC‐method in present studies has yielded new information about the cytogenetics of CLL. Although most of the interphase cells from patients with B‐CLL were positive for B‐cell markers, many of the mitotic cells turned out to be T cells, supporting the notion that the cells studied by conventional chromosome analysis are often non‐neoplastic T cells. In some B‐CLL cases with normal karyotype in the conventional chromosome study, however, most of the mitotic cells were B cells, indicating that neoplastic B cells may also have a normal karyotype. The patient with T‐CLL had normal karyotype even though most of the mitoses were T cells. The chromosome abnormalities found were restricted to cells with light chain clonality. Our results show that clonal chromosome abnormalities do occur in neoplastic B cells of patients with B‐CLL.