Premium
Regrowth of granulocyte‐macrophage progenitor cells (GM‐CFC) in suspension cultures of bone marrow depleted of GM‐CFC with 4‐hydroperoxycyclophosphamide (4‐HC) *
Author(s) -
Beran Miloslav,
Zander Axel R.,
Andersson B. S.,
NcCredie Kenneth B.
Publication year - 1987
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1987.tb00740.x
Subject(s) - bone marrow , granulocyte , progenitor cell , granulocyte macrophage colony stimulating factor , macrophage , suspension culture , immunology , haematopoiesis , cfu gm , suspension (topology) , chemistry , pathology , medicine , cell culture , biology , microbiology and biotechnology , stem cell , in vitro , biochemistry , cytokine , genetics , mathematics , homotopy , pure mathematics
The hematopoietic capacity of bone marrow treated with 4‐hydroperoxyclopho‐sphamide (4‐HC) was studied using long‐term suspension cultures. It was shown that, in the presence of a previously established marrow adherent cell layer (ACL), the 4‐HC treated bone marrow, deprived of GM‐CFC, generates GM‐CFC in long‐term suspension cultures in vitro. The kinetics of GM‐CFC reappearance in in vitro cultures indicate their origin from more primitive progenitors rather than from surviving GM‐CFC. In the presented modification, the suspension culture system is useful in evaluating the transplantation potential of bone marrow purged in vitro with chemo‐therapeutic or biologic agents.