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Characterization of lymphocyte subpopulations in human cord blood using the immunogold staining technique
Author(s) -
Fatunde Oluyemisi J.,
Kaplan Sandra S.,
Lochner Janet B.,
Penchansky Lila
Publication year - 1987
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1987.tb00157.x
Subject(s) - calla , cord blood , lymphocyte , immunogold labelling , monoclonal antibody , umbilical cord , immunology , biology , staining , pathology , bone marrow , cord , peripheral blood lymphocyte , medicine , antibody , surgery
Lymphocyte subpopulations in human cord blood have been examined using monoclonal antibodies, visualized with immunogold. The proportions of T 11 , T 4 , T 8 , and B 1 , cells in cord blood are very similar to values in adult peripheral blood. Some evidence of lymphocyte immaturity in cord blood is suggested by the presence of 12% CALLA‐positive cells and the sum of T 4 and T 8 cells significantly exceeding the number of T 11 cells; however, there were no TdT‐positive cells. The presence of CALLA‐positive lymphocytes in normal cord blood should be borne in mind when investigating blood smears from neonates for congenital leukemia.