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Colony inhibiting factor in mature granulocytes from normal individuals and patients with chronic myeloid leukemia
Author(s) -
Bøyum Arne,
Løvhaug Dagfinn,
Kolstø Anne Brit,
Helgestad Jon,
Melby Tone
Publication year - 1987
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1987.tb00005.x
Subject(s) - colony stimulating factor , lactoferrin , in vitro , myeloid leukemia , granulocyte , myeloid , biology , immunology , microbiology and biotechnology , andrology , haematopoiesis , medicine , biochemistry , stem cell
Inhibitory activity in extract from human blood granulocytes was tested on gran‐ulocyte‐macrophage colony formation in vitro. The inhibition depended on the type of serum used. With mouse BMC and FCS in the cultures, extract corresponding to 2.5 times 10 4 granulocytes/ml reduced the colony number by 35%, and extract from 2 times 10 5 cells caused maximal inhibition (80–90%). With HS and mouse BMC the colony number was reduced by only 11–12%, but stronger inhibition (55%) was observed when the serum concentration was reduced. With both types of sera the total cell number per culture plate was reduced relatively more than the colony number. Human GM‐CFC were as sensitive as mouse GM‐CFC, and extract from CML granulocytes inhibited less (p < 0.01) than extract from normal cells. Biochemical studies indicated that the inhibitor is a protein with a molecular weight of 30–60000. Lactoferrin, a putative inhibitor of CSF production, did not inhibit spontaneous or CSF‐induced colony formation in these studies.