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Characterization of normal and regenerating bone marrow cells with a panel of monoclonal antibodies
Author(s) -
KluinNelemans Hanneke C.,
Bolhuis R. L. H.,
Lowenberg B.,
Campana D.,
Sizoo Willemijn
Publication year - 1986
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1986.tb02653.x
Subject(s) - bone marrow , calla , lymphoma , pathology , chemotherapy , monoclonal antibody , medicine , monoclonal , staining , lymphocyte , antibody , immunology
To obtain baseline data for the diagnosis of residua] leukaemia or lymphoma in bone marrow (BM), BM samples of 12 normal adults, 6 patients treated with chemotherapy for solid tumours and 6 patients treated with chemotherapy for leukaemia or lymphoma during remission were assessed with a panel of monoclonal antibodies by fluorescence microscopy immediately following chemotherapy. A large variability between normal individuals was found concerning the percentages of T lymphocytes and T cell subsets, B lymphocytes, HLA‐DR* and OKT104 cells, due to variation in BM composition and, presumably, peripheral blood contamination. The regenerating BM cells also showed a large variability and, in addition, sometimes very high percentages (up to 19%) of CALLA +, cu'sjr, cu + KV and TdT+ cells were found. The interpretation of data on individual BM samples of patients assessed for leukaemia or lymphoma should therefore be related to: (1) the amount of contaminating peripheral blood cells; (2) the cellular composition of the BM samples as determined by May‐Grunwald Giemsa staining; (3) baseline data of age‐matched normal controls and of regenerating BM of patients without haematological diseases.