Premium
Nucleolus‐associated J chains in myeloma cells
Author(s) -
Kanoh Tadashi,
Ohnaka Tadashi,
Takamatsu Teruyuki,
Ohno Tatsuharu,
Uchino Haruto,
Horiguchi Yuji
Publication year - 1986
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1986.tb02635.x
Subject(s) - nucleolus , immunoglobulin light chain , immunoelectron microscopy , multiple myeloma , bence jones protein , cytoplasm , electron microscope , nucleus , bone marrow , myeloma protein , chemistry , pathology , biology , antibody , microbiology and biotechnology , medicine , immunohistochemistry , physics , immunology , optics
Bone marrow aspirates from 10 patients with multiple myeloma (6 IgG myeloma, 3 IgA myeloma, 1 Bence Jones myeloma) were examined for nucleolus‐associated localization of J chains. On light microscopy using the PAP method, almost all or a significant number of myeloma cells exhibited intranuclear spots stained with anti‐J chains in 3 (IgG myeloma) out of the 8 cases positive for cytoplasmic J chains. In contrast, the nucleus of myeloma cells examined was constantly negative for anti‐heavy and ‐light chains. Immunoelectron microscopically, J chain was identified as dicrete round electron‐dense precipitates, corresponding to the whole nucleolus, and as sparsely distributed, small electron‐dense deposits in the nucleus. In addition, some connections were found between nuclear and nucleolar electron‐dense precipitates. Several possible explanations have been proposed to account for this localization of J chains.