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The effect of α and γ‐interferon on proliferation and production of IgE and β 2 ‐microglobulin in the human myeloma cell line U‐266 and in an α‐interferon resistant U‐266 subline
Author(s) -
Brenning G.,
Jernberg H.,
Gidlund M.,
Sjöberg O.,
Nilsson K.
Publication year - 1986
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1986.tb02313.x
Subject(s) - immunoglobulin e , cell culture , in vitro , recombinant dna , secretion , interferon , biology , cell growth , beta 2 microglobulin , microbiology and biotechnology , immunology , antibody , endocrinology , biochemistry , gene , genetics
An IFN‐resistant subline (U‐266 r α ) was established from the IFN‐α‐sensitive myeloma cell line U‐266 by subculturing U‐266 cells with increasing doses of INF‐α. The U‐266 r α secreted IgE at a higher rate than the U‐266 (7.2 × 10 −13 g/c/8 h as compared to 3.3 × 10 −13 g/c/8 h). The 2 cell lines were found to be equally high producers of β 2 m (9.2 and 9.6 × 10 −13 g/c/8 h). The U‐266 produced 2.9 times less IgE and 5 times more β 2 m compared to the initial production rates at establishment. INF‐α and recombinant IFN‐αM 2 (rIFN‐α 2 ) inhibited proliferation and concomitantly decreased the rate of IgE and β 2 m secretion in U‐266 but not in U‐266 IFN r α , which in contrast was slightly stimulated by IFN‐α with respect to growth, IgE and β 2 m secretion. In addition, IFN‐α at a concentration of 100 U/ml was shown to decrease the IgE and β 2 m production without exerting more than minimal cytotoxicity on U‐266 cells. No antiproliferative effect was found for IFN‐γ or recombinant IFN‐γ (rIFN‐γ) on either of the 2 cell lines. IFN‐γ and rIFN‐γ were, however, found to stimulate the production of β 2 m. Our results show that the U‐266 and the derived IFN‐α‐resistant subline can be used as models for studying some of the biological effects of IFN‐α and ‐γ in vitro. The clinical implications of these in vitro results, in particular the usefulness of serum determinations of immunoglobulin and β 2 m concentrations for monitoring the tumor cell mass, are discussed.