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Effects of a hemoregulatory peptide (HP5b) on erythroid and myelopoietic colony formation in vitro
Author(s) -
Kreja Ludwika,
Hågå Per,
MullerBérat Nicole,
Laerum Ole Didrik,
Sletvold Olav,
Paukovits Walter R.
Publication year - 1986
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1986.tb01777.x
Subject(s) - myelopoiesis , peptide , haematopoiesis , in vitro , biology , inhibitory postsynaptic potential , chemistry , immunology , microbiology and biotechnology , biochemistry , endocrinology , stem cell
A hemoregulatory peptide (HP5b) associated with mature human granulocytes has been investigated for inhibitory effects on murine hemopoietic stem cells in vitro. Both highly purified peptide from natural sources and a synthetic analog peptide have been investigated in parallel. Strong inhibitory effects were found on myelopoietic colony formation in the dose range 10 −13 ‐10 −5 mol/l. On exceeding this dose, the inhibitory effect disappeared. Moderate to slight inhibitory effects on erythroid colony formation (BFU‐E and CFU‐E) from adult animals were only seen in 1000 x the optimal doses for myelopoiesis. No effect was found on CFU‐E from fetal liver. The peptide thus has a selective effect on myelopoiesis in a certain dose range. A regulatory mechanism for the peptide on hemopoiesis is proposed.