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Assessment of the role of an immunofluorescent terminal deoxynucleotidyl transferase kit
Author(s) -
Dunbar D. R.,
Smith C.,
Thornton J. A.,
Waters H. M.,
Hyde K.,
Delamore I. W.
Publication year - 1985
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1985.tb02815.x
Subject(s) - terminal deoxynucleotidyl transferase , tunel assay , transferase , microbiology and biotechnology , terminal (telecommunication) , chemistry , pathology , biology , medicine , biochemistry , computer science , immunohistochemistry , enzyme , telecommunications
The laboratory use of a commercial kit for the immunofluorescent measurement of terminal deoxynucleotidyl transferase (BRL Terminal Deoxynucleotidyl Transferase Immunofluorescent Assay System) has been investigated. The precision of the assay system with stored patient material and its performance with regard to ease of microscopic examination were assessed. The intensity of the fluorescence obtained was found to vary. However, the precision of the assay system on fixed slides stored at ‐30°C was satisfactory; the method simple, and results obtainable in a relatively short space of time.